BioImaging Utrecht will have a winter meeting on Wednesday 21st of February from 10:00 to 11:00. It will be followed by an IMARIS workshop at the Stratenum (MCR). Georgia Golfis will outline the program at 11:00 -12:30 (Rm STR3.108) and she will train groups to work with own datasets in the afternoon. The next day, Thursday the 22nd a workshop to all Imaris users will be given (RM STR 2.106). Registration for the workshop is required by mail to Livio (L.Kleij@umcutrecht.n). Highly recommended for Imaris users!! (Announcement PDF).
Unfortunately, the TOKAI hit lens heater is broken again. We are in contact with the Japanese supplier but it may take some time for it to be repaired (it needs to be sent to Japan). We have imaged live cells without the heater overnight without any problems, yet the temperature at the lens spot will be several degrees below the set temperature. Contact CCI staff if you intent to install the hairdryer for additional heating.
The TOKAI hit objective heater is complete again. Be careful when changing objectives when the velcro is around it. The connecting wires can be dislodged from the connectors by these fast movements. Also, the aluminium inner surface is fragile be carefull when you wrap te velcro around an objective.
The objective heater for live cell imaging on the NIKON A1R-STORM system is broken. The thermocouple is being replaced. For short term time lapse (and vibration insensitive imaging) you can use the hair dryer to warm the lower part of the microscope.
We are missing the 3.5 cm dish holder that allows clamping a 3.5 dish onto the galvano stage of the SPE-2 confocal. Please return if you used it, other people need to use it!! It should be stored in the left cabinet behind the SPE-2 above the freezer.
At the CCI office, a slide and photo scanner (link) was installed to scan negatives, overviews of histology slides and exposed ECL films. Please contact Richard if you want to use it.
The BioImaging Utrecht community (link) has a number of events planned that are highly recommended.
Hardware Microscopy demonstrations.
Demonstration of the re-scan confocal (link) at the UMCU was only last week (contact Corlinda if you want to know how good it was). Additional product demonstrations are planned:
29th of September:
CeniBra / Yokogawa representatives will give talks on a spinning disk desktop machine, the CQ1 (link) that should be well suited to image and analyse organoid containing volumes.
4th of October
Leica will host lectures (link) on their high end imaging systems on the 4th of October (especially their new DMi8S TIRF live imaging setup) – Lukas Kapitein and Philip Tinnefeld will lecture on TIRF use in their labs, no doubt spectacular work will be shown. Hands-on workshops will continue the next day.
The Utrecht Image Contest (link)
This year Utrecht microscopists are again invited to send in their most beautiful and/or artistic image in jpg format to compete for the prestigious title of the best Utrecht Microscopy Image in the Electron Microscopy or Fluorescence microscopy categories. In addition, a printout by pArt of Science (link) and a prize of 200 Euro (provided by Carl Zeiss) can be won. Please supply image with microscope information and a brief scientific description to : email@example.com
ImageJ/FIJI workshop (BioImaging Utrecht_ImageJ beginners 2017)
At this workshop, image analyses experts in the Utrecht imaging community will train you to learn using ImageJ/FIJI and give you an idea how to start analyzing (microscopy) images in general. Highly recommended for microscopists. Good to realize that image analyses will usually make your image acquisition better and will help you focus on the actual measurement of imaging process. Registration deadline October 1st (ImageJ_beginners_registration form 2017).
We obtained a 5x Plan ApoChromat lens for the Zeiss NLO-LSM880 together with a universal stage adapter from PECON (KM) that fits 85.5x 127.75 mm culture plates (i.e. 6, 12,24,48,96,384 well culture plates). Automated live cell multiwell analyses at 37 degrees C will be possbile but without CO2 and humidity control for now. The old slider holder is still available to accommodate 25×75 mmm slides, labtek slides and culture dishes (35, 60 mm diameter). Many thanks to Bart Spee and Louis Penning (DGK GZD) who made these accessories available to the equipment at the CCI.
The attachment of the different adapters for the TOKAI hit heating chamber on the A1R NIKON / STORM microscope has been changed. Magnets have been glued onto the MCL-piezo stepper adapter to snap sample holders by pairing magnets. Place a sample holder and carefully put the supplied separate 12 mm diameter plate magnets over the front-left and back-right corners.
The theme of the upcoming spring meeting of the BioImaging Utrecht society is: time resolved 3D imaging of large samples. Please find the announcement poster (here) to print and display. Presentations on using light sheet microscopy in brain development and introducing Anne Rios’ work on imaging the 3D architecture of developing breast cancers.
Place: David de Wied building Rm M2.01
Date and time: Tuesday 16th of May at 15:00.