New STORM analyses software to perform peak detection by multifitting procedure (described in: Babcock et al 2012 Opt Nanoscopy 1:6 ) is now implemented on our machines (workstation and acquisition PC).
The STORM module of the NIKON A1R/STORM system has been updated. Now 3D color chromatic aberration correction is included in the module and post acquisition filtering of peak minima and maxima has been implemented. The workstation software is upgraded as well.
We had a training session at NIKON to work with the wizard creator ‘JOBS’ within the NIS software. It is aimed to easily guide people through an imaging session. Especially multiwell plate definition, random position imaging within wells, autofocus, different image capture for different wells can be predefined and structured. Of course, users still need to know how to tweak the system to get good images but it may turn out to become a popular tool for our facility.
Tuesday (21st of January), the BioImaging Utrecht community will have an open meeting on nanoscopy. Join to hear the latest on superresolution. Flyer can be obtained here.
An update of the A1R software and the STORM software has been performed, functionally the 4.2 NIS version is identical (bugfixes) but the STORM module has improved possibilities for centroid determination.
We are going to discontinue the service to provide secundary fluorescently labelled antibodies. It was useful to maintain a relatively fresh stock that is continuously probed for functionality, this way users did not need to worry about out-of-date fluorescent antibodies. However, this service is too expensive to keep, and if users keep stocks as 50% glycerol solutions in -20 C freezers long term storage is very stable. The problem of drying and getting aggregates of non-functional antibodies is minimised by this procedure. Our advice is to generate a stock of secundary antibodies and share this among department members (one or two persons responsible for the ordering, aliquotting and distribution clarifies cost streams and inventory overview). A handy tool to keep track of inventories is supplied as freeware by Quartzy.
To help users I have developed a HTML app (see QRcode below, for now- we are still in testing phase) to log mic setings and send to the user by mail, booking is also included and a checklist to guide STORM imaging is in there as well. Handy for keeping track of some settings you would like to have available before you start a new imaging experiment, making a rule in mailing programs can keep these mails toghether, if you prefer. Native app for Android, Apple and Windows will follow if the app functions well. We noted that some users show personal info on the booking site, beware that this is a public site and anyone can see this (editting is restricted to users though).
QRcode for CCI -app