C C I – Centre for Cell Imaging
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Spectral Viewer (Life Technologies)
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Scripts IJ/Cell Profiler
Fluorescence microscopy at VMed Utrecht University
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Equipment
Matching microscope to your application at the CCI
NIKON A1R/TIRF/STORM
OMX-V4 Blaze
Zeiss LSM 880 NLO
Leica SPE-II – DMI4000
Olympus BX60 Fluorescence – Histology
Leica S6D Preparation Microscope
LEICA CRYOSTATE 3050 AND 3050S
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Introduction fluorescence microscopy
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Software
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Scripts IJ/Cell Profiler
ImageJ Macros
Contact Sheet for .lif files
Calculate Edu+ (ch02) cell in stack of DAPI nuclei (ch01)
2 CH intensity measurement homogeneity in grid
Mouse listener to draw ROI on DIC/Ch02 and measure in Ch01
Cell Profiler Scripts
Publications