On Tuesday May15th from 15:00-17:00 lectures by Lukas Kapitein and Nalan Liv will be given on Light Sheet Microscopy (Sibarita style) and Correlative live-cell and 3D electron microscopy of sub cellular membrane compartments respectively.
Location: HUBRECHT Auditorium
In addition, microscopy demos are organized. A Luxendo inVi-SPIM lightsheet microscope will be at the Hubrecht from the 15th to the 25th of May. Contact Anko de Graaff if you want to try the machine with your samples (email@example.com) .
A second system that will be demonstrated is the GEH Deltavision Ultra, it will be in the UMCU CMC (H02.1.22 -Stratenum ) from 14th to the 17th of May. Contact, Allan Hemmingsen of GEH (firstname.lastname@example.org) to try this system.
A newly developed joystick is installed on the OMX-V4, it allows navigating through a sample, mark and start acquisition/mosaic. It is a bit fast but with practice will help you find the best cells more efficiently.
Few new features are implemented during the last service visit (for example: laser power tuning), ask CCI staff to inform you before working on the machine. Again, be sure to keep the OTFs of April 2018 for the reconstructions.
The TOKAI HIT heater for lenses on the NIKON system is repaired. Please be careful wrapping the foil around the lenses, the fit should be tight but do not pull the wiring or deform the aluminium foil while wrapping.
The OMX system has been serviced and new OTFs have been generated. The current OTF set that is selected in SoftWoRx should be used for reconstructions, do not change it!
BioImaging Utrecht will have a winter meeting on Wednesday 21st of February from 10:00 to 11:00. It will be followed by an IMARIS workshop at the Stratenum (MCR). Georgia Golfis will outline the program at 11:00 -12:30 (Rm STR3.108) and she will train groups to work with own datasets in the afternoon. The next day, Thursday the 22nd a workshop to all Imaris users will be given (RM STR 2.106). Registration for the workshop is required by mail to Livio (L.Kleij@umcutrecht.n). Highly recommended for Imaris users!! (Announcement PDF).
Unfortunately, the TOKAI hit lens heater is broken again. We are in contact with the Japanese supplier but it may take some time for it to be repaired (it needs to be sent to Japan). We have imaged live cells without the heater overnight without any problems, yet the temperature at the lens spot will be several degrees below the set temperature. Contact CCI staff if you intent to install the hairdryer for additional heating.
The TOKAI hit objective heater is complete again. Be careful when changing objectives when the velcro is around it. The connecting wires can be dislodged from the connectors by these fast movements. Also, the aluminium inner surface is fragile be carefull when you wrap te velcro around an objective.
The objective heater for live cell imaging on the NIKON A1R-STORM system is broken. The thermocouple is being replaced. For short term time lapse (and vibration insensitive imaging) you can use the hair dryer to warm the lower part of the microscope.
We are missing the 3.5 cm dish holder that allows clamping a 3.5 dish onto the galvano stage of the SPE-2 confocal. Please return if you used it, other people need to use it!! It should be stored in the left cabinet behind the SPE-2 above the freezer.
At the CCI office, a slide and photo scanner (link) was installed to scan negatives, overviews of histology slides and exposed ECL films. Please contact Richard if you want to use it.