We obtained a 5x Plan ApoChromat lens for the Zeiss NLO-LSM880 together with a universal stage adapter from PECON (KM) that fits 85.5x 127.75 mm culture plates (i.e. 6, 12,24,48,96,384 well culture plates). Automated live cell multiwell analyses at 37 degrees C will be possbile but without CO2 and humidity control for now. The old slider holder is still available to accommodate 25×75 mmm slides, labtek slides and culture dishes (35, 60 mm diameter). Many thanks to Bart Spee and Louis Penning (DGK GZD) who made these accessories available to the equipment at the CCI.
The attachment of the different adapters for the TOKAI hit heating chamber on the A1R NIKON / STORM microscope has been changed. Magnets have been glued onto the MCL-piezo stepper adapter to snap sample holders by pairing magnets. Place a sample holder and carefully put the supplied separate 12 mm diameter plate magnets over the front-left and back-right corners.
The theme of the upcoming spring meeting of the BioImaging Utrecht society is: time resolved 3D imaging of large samples. Please find the announcement poster (here) to print and display. Presentations on using light sheet microscopy in brain development and introducing Anne Rios’ work on imaging the 3D architecture of developing breast cancers.
Place: David de Wied building Rm M2.01
Date and time: Tuesday 16th of May at 15:00.
The Bio-Imaging Utrecht community organizes an ImageJ/FIJI workshop for experienced users on Tuesday the 4th of April. Previous sessions were very well appreciated and they allow you to interact with experts in IJ coding. You can learn image analyses automation and the experts can help you find, tweak and apply the proper plugin(s) for you specific question. It is highly advised for microscopists who use FIJI but have problems overseeing the large analyses possibilities offered by the platform. Register using the form attached here (deadline 17th March)
On Tuesday 28th of February to Wednesday the 1st of March the OMX will be unavailable. A sCMOS PCO camera will be replaced and the system will be fully re-aligned.
The OMX has been serviced and new OTFs have been made. We recommend using these. A strange bug in the acquisition software causes the 405 nm laser line (1st channel) to present wrong values, i.e. intensity % of the laser in the drop-down list do not represent the actual values. The desktop background picture of the PC instructs you restore the right interface (in 3 clicks).
Our new 63x lens (NA 1.2) is working from within ZEN black. A slider becomes available when you choose the objective that allows adjusting the depth correction of the lens. It is very nice to get a better psf at the depth of your most interesting feature. Good when you plan to deconvolve images post acquisition.
Finally, we have curtains around the SPEII to seal the microscope space from the rest of the lab. This way we now can work normally in the wetlab again with the lights on.
We are very grateful to the faculty to expand the Zeiss LSM880 NLO functionality with a hi NA (1.2, 63x) water immersion objective with an automated correction collar. This will allow high resolution imaging on the machine that is currently equipped with a 20x (NA 0.8) and a 32x (NA 0.85) objective. We are waiting for a connector unit to get it working and will be testing its performance shortly afterwards.
A new 20x Plan Achromat VC objective (air) has arrived and is installed on the NIKON A1R/STORM machine. The multi immersion objective is removed from the turret but will remain available for users (ask CCI staff if you prefer to use it). The new objective is more widely applicable, has a working distance of 1 mm, a NA of 0.75 and should work well with the perfect focus system. We will test it in the coming period.