Category Archives: Leica SPE-II

Leica SPE2 40x HCX oil objective defect

The 40x oil on the Leica SPE-II CLSM showed severe chromatic aberration in Z for UV fluorescence. The objective is removed from the system and its aberration is being validated on another Leica machine in the UU campus. We probably need to repair or replace it. Our objectives are very costly and vulnerable, please handle them with care. A microcourse on how to prevent lens damage is given by dr. Waters (link). We notice a great number of scratches on the top beveled end of all lenses, please realize that these are often generated by collisions to the bottom edge of a sample holder. Please make sure lenses are lowered before rotating the turret and realize that moving the stage at some point will get the lens too close to the sample holder edge.

Walking directions during reboot of the CCI labs

Working on the Leica SPE2:

Entry: General entrance AG East 1 st floor, direct right turn to access the Confocal room O.161, knock before entry and apply to the hygiene rules that are supplied. A second person can be present in the room yet distance of 1.5 m should be kept at all times.

Exit: Leave following the same path.

Working on the Olympus histology microscope:

Entry: General entrance AG east 1 st floor, direct right turn to access the O.161, knock before entering and turn left and avoid coming close to the people working here. Only one person on the microscope.

Exit: Leave following the same path or move through the ML-2 lab space if someone enters when you are still in the narrow room

Working on the OMX:

Entry: General entrance AG east 1st floor, enter ML-2 lab from the corridor. Knock before entering, max 1 person in the OMX rooms (laser station room, workstation room). The corridor in the ML-2 space is co-occupied, so be careful to move through it and notify others that you are coming though.

Exit: same route.

Working on the NIKON:

Entry: General entrance AG east 1st floor, enter ML-2 lab from the corridor. Knock before entering, max 1 person in the NIKON-A1R room. The corridor in the ML-2 space is co-occupied, so be careful to move through it and notify others that you are coming though.

Exit: same route.

Working on the Zeiss LSM 880 NLO:

Entry: General entrance AG east 1st floor, enter ML-2 lab from the corridor. Knock before entering, max 1 person in the Zeiss LSM880 room. The corridor in the ML-2 space is co-occupied, so be careful to move through it and notify others that you are coming though.

Exit: same route.

April 4: ImageJ Advanced workshop

The Bio-Imaging Utrecht community organizes an ImageJ/FIJI workshop for experienced users on Tuesday the 4th of April. Previous sessions were very well appreciated and they allow you to interact with experts in IJ coding. You can learn image analyses automation and the experts can help you find, tweak and apply the proper plugin(s) for you specific question. It is highly advised for microscopists who use FIJI but have problems overseeing the large analyses possibilities offered by the platform. Register using the form attached here (deadline 17th March) 

Leica SPEII vibration issue

Image distortions due to vibrations on the system were reported last week. We elevated the vibration insulator silicon supports and temporarily solved the problem. New insulators will be placed during the next Leica service. Please notify us when stripe patterns appear in your images.

Leica SPE-II shifted in room O-101

We have an old building that is leaky, a serious water spill occurred when the last storm hit the east wing of the Androclus building. The equipment (the SPEII) was not damaged due to the swift action of Martijn Moolenaar (thanks again). Apparently, the rain leaked into the building at multiple departments and it is a major concern of the maintenance department. Although patch-type preventive measures are in progress, recurrence of the flooding cannot be ruled out. We urge users to  notify CCI staf asap when it occurs again.

To minimize damage due to such a recurring accident we moved the SPEII to the east wall of the room and lifted all wiring from the floor. The machine is in full operation again.

Clearly, another save location for the SPEII in the tightly packed CCI is high on our agenda.

DATA storage testing

A StorSimple backup system has been  implemented by ITS.  We have tested it for a couple of months and it seems to perform well. The extra network drive is available on all imaging machines (except for the Zeiss NLO system, that will be connected to the net in the coming weeks) and can be found at O:\Research\CCI\#equipment\##user##.  Writing on the drive  can only be done on our machines, users will be able to read only from these drives so data will be available but secured. We are implementing a secure logon system for users to read data from this drive.

 

Leica SPE-II 40x Objective

The new 40x objective (HC PL APO CS2 NA1.3, oil immersion) has been equipped with the correct condenser prisms some time ago, but it was not mentioned here. So the DIC and fluorescence is fully upgraded on the machine. Please realize the greater sensitivity of the new objective. Intensity measurements acquired before June with the 40x will have lower values than the current 40x.