Category Archives: NIKON A1R/TIRF/STORM

Lens heater on NIKON A1R/STORM again failing

Unfortunately, the TOKAI hit lens heater is broken again. We are in contact with the Japanese supplier but it may take some time for it to be repaired (it needs to be sent to Japan). We have imaged live cells without the heater overnight without any problems, yet the temperature at the lens spot will be several degrees below the set temperature. Contact CCI staff if you intent to install the hairdryer for additional heating.

April 4: ImageJ Advanced workshop

The Bio-Imaging Utrecht community organizes an ImageJ/FIJI workshop for experienced users on Tuesday the 4th of April. Previous sessions were very well appreciated and they allow you to interact with experts in IJ coding. You can learn image analyses automation and the experts can help you find, tweak and apply the proper plugin(s) for you specific question. It is highly advised for microscopists who use FIJI but have problems overseeing the large analyses possibilities offered by the platform. Register using the form attached here (deadline 17th March) 

New NIKON 20x Plan Achromat objective

A new 20x Plan Achromat VC objective (air) has arrived and is installed on the NIKON A1R/STORM machine. The multi immersion objective is removed from the turret but will remain available for users (ask CCI staff if you prefer to use it). The new objective is more widely applicable, has a working distance of 1 mm, a NA of 0.75 and should work well with the perfect focus system. We will test it in the coming period.

Update NIS elements and STORM module

Today, the NIS elements software on the NIKON A1R/STORM system has been updated to the newest version for the NIS suite (4.5) and the STORM module (4.1). In STORM,  Z stacking features, fiducial marker integration and sequential recordings on identical positions can be performed, potentially on regions in  live cells. In addtition, the 405 laser was re-aligned and set at a higher level in the startup script to allow better imaging in the UV region. Users should take care that this laser setting is different from their previous sessions.