We obtained a 5x Plan ApoChromat lens for the Zeiss NLO-LSM880 together with a universal stage adapter from PECON (KM) that fits 85.5x 127.75 mm culture plates (i.e. 6, 12,24,48,96,384 well culture plates). Automated live cell multiwell analyses at 37 degrees C will be possbile but without CO2 and humidity control for now. The old slider holder is still available to accommodate 25×75 mmm slides, labtek slides and culture dishes (35, 60 mm diameter). Many thanks to Bart Spee and Louis Penning (DGK GZD) who made these accessories available to the equipment at the CCI.
The Bio-Imaging Utrecht community organizes an ImageJ/FIJI workshop for experienced users on Tuesday the 4th of April. Previous sessions were very well appreciated and they allow you to interact with experts in IJ coding. You can learn image analyses automation and the experts can help you find, tweak and apply the proper plugin(s) for you specific question. It is highly advised for microscopists who use FIJI but have problems overseeing the large analyses possibilities offered by the platform. Register using the form attached here (deadline 17th March)
Our new 63x lens (NA 1.2) is working from within ZEN black. A slider becomes available when you choose the objective that allows adjusting the depth correction of the lens. It is very nice to get a better psf at the depth of your most interesting feature. Good when you plan to deconvolve images post acquisition.
We are very grateful to the faculty to expand the Zeiss LSM880 NLO functionality with a hi NA (1.2, 63x) water immersion objective with an automated correction collar. This will allow high resolution imaging on the machine that is currently equipped with a 20x (NA 0.8) and a 32x (NA 0.85) objective. We are waiting for a connector unit to get it working and will be testing its performance shortly afterwards.
The Zeiss LSM880 NLO has been installed. We will be instructed to use it in the coming week and the machine performance will be evaluated afterwards. The multiphoton imaging machine is excellently suited to perform microscopy on thick samples.
When you want to do in vivo mouse imaging on the machine, please contact dr. Nienke Vrisekoop (N.Vrisekoop@umcutrecht.nl) at the WKZ/LTI, who is the expert and coordinator on the machine for this work. Be aware that you need to prepare the proper licences (GMO and DEC) to perform these experiments.
Contact CCI staff to request imaging of other sample types with the LSM880.
Due to the renewal of equipment, we will reoganize the CCI drastically.
First, the space that will host the multiphoton system is being prepared. This is a collaboration with the WKZ, see earlier posts about this. This system will be placed in room O122. The co-ordinator of work on this machine will initially be Nienke Vrisekoop. We will facilitate instruction, authorisation and general surveillance of the device.
In preparation of this, the Olympus microscope has been moved to O123A, see an earlier post about this.
Second, we need to fit the OMX system within our limited space which has proven to be a major task. Currently, we plan to move the SPE2 system to the wetlab (room O101) are reserve it for maximally ML1 work. The OMX will then occupy room O124 and O124A.
To do this reorganisation and restore arrears of maintenance on the airconditioning units, we will face disturbance in the CCI day-to-day business in the coming weeks/months. Watch this space for updates.
Regrettably, the Biorad Multiphoton system is no longer in service. We are going to move the femtosecond Tsunami laser to the Biology Department where Corette Wieringa will be building it into an existing photomanipulation system to give it yet another functional use.
We are negotiating with Nienke Vrisekoop, Kikki Tesselaar and Leo Koenderman from the UMCU to place a Zeiss LSM7 series multiphoton system (NLO) in room O124A. Follow this space for updates.