BioImaging Utrecht will have a winter meeting on Wednesday 21st of February from 10:00 to 11:00. It will be followed by an IMARIS workshop at the Stratenum (MCR). Georgia Golfis will outline the program at 11:00 -12:30 (Rm STR3.108) and she will train groups to work with own datasets in the afternoon. The next day, Thursday the 22nd a workshop to all Imaris users will be given (RM STR 2.106). Registration for the workshop is required by mail to Livio (L.Kleij@umcutrecht.n). Highly recommended for Imaris users!! (Announcement PDF).
The Bio-Imaging Utrecht community organizes an ImageJ/FIJI workshop for experienced users on Tuesday the 4th of April. Previous sessions were very well appreciated and they allow you to interact with experts in IJ coding. You can learn image analyses automation and the experts can help you find, tweak and apply the proper plugin(s) for you specific question. It is highly advised for microscopists who use FIJI but have problems overseeing the large analyses possibilities offered by the platform. Register using the form attached here (deadline 17th March)
The OMX has been serviced and new OTFs have been made. We recommend using these. A strange bug in the acquisition software causes the 405 nm laser line (1st channel) to present wrong values, i.e. intensity % of the laser in the drop-down list do not represent the actual values. The desktop background picture of the PC instructs you restore the right interface (in 3 clicks).
Our new 63x lens (NA 1.2) is working from within ZEN black. A slider becomes available when you choose the objective that allows adjusting the depth correction of the lens. It is very nice to get a better psf at the depth of your most interesting feature. Good when you plan to deconvolve images post acquisition.
This week the filter drawer changer that was locked has been repaired. The system was serviced and softWoRx packages on the Windows master acquisition station and the workstations have been updated.
Today, the NIS elements software on the NIKON A1R/STORM system has been updated to the newest version for the NIS suite (4.5) and the STORM module (4.1). In STORM, Z stacking features, fiducial marker integration and sequential recordings on identical positions can be performed, potentially on regions in live cells. In addtition, the 405 laser was re-aligned and set at a higher level in the startup script to allow better imaging in the UV region. Users should take care that this laser setting is different from their previous sessions.
We are experiencing problems with our netstorage. The central storage is currently not available so you will not be able to upload or download data. The data are secured and will be available again soon. Watch this blog for updates.
Matlab is now available as a campus licence. We installed the newest version of Matlab on our windows workstations (the NIS workstation, the Imaris Workstation and Richard’s PC). The IT department will inform you how to install MATLAB on your computer via its licence manager.
We now have a workstation that runs Imaris 8 (full licence) at the CCI. Experienced users are welcome to use it, it can be booked in the on-line reservation system. We will be trained by Imaris application specialists in the coming months to offer best support for this excellent image analysis package.
Esther has updated the Leica handout for software and hardware changes in the last year, please find the new version on the equipment page or here