We obtained a 5x Plan ApoChromat lens for the Zeiss NLO-LSM880 together with a universal stage adapter from PECON (KM) that fits 85.5x 127.75 mm culture plates (i.e. 6, 12,24,48,96,384 well culture plates). Automated live cell multiwell analyses at 37 degrees C will be possbile but without CO2 and humidity control for now. The old slider holder is still available to accommodate 25×75 mmm slides, labtek slides and culture dishes (35, 60 mm diameter). Many thanks to Bart Spee and Louis Penning (DGK GZD) who made these accessories available to the equipment at the CCI.
The attachment of the different adapters for the TOKAI hit heating chamber on the A1R NIKON / STORM microscope has been changed. Magnets have been glued onto the MCL-piezo stepper adapter to snap sample holders by pairing magnets. Place a sample holder and carefully put the supplied separate 12 mm diameter plate magnets over the front-left and back-right corners.
On Tuesday 28th of February to Wednesday the 1st of March the OMX will be unavailable. A sCMOS PCO camera will be replaced and the system will be fully re-aligned.
The OMX has been serviced and new OTFs have been made. We recommend using these. A strange bug in the acquisition software causes the 405 nm laser line (1st channel) to present wrong values, i.e. intensity % of the laser in the drop-down list do not represent the actual values. The desktop background picture of the PC instructs you restore the right interface (in 3 clicks).
Our new 63x lens (NA 1.2) is working from within ZEN black. A slider becomes available when you choose the objective that allows adjusting the depth correction of the lens. It is very nice to get a better psf at the depth of your most interesting feature. Good when you plan to deconvolve images post acquisition.
Finally, we have curtains around the SPEII to seal the microscope space from the rest of the lab. This way we now can work normally in the wetlab again with the lights on.
We are very grateful to the faculty to expand the Zeiss LSM880 NLO functionality with a hi NA (1.2, 63x) water immersion objective with an automated correction collar. This will allow high resolution imaging on the machine that is currently equipped with a 20x (NA 0.8) and a 32x (NA 0.85) objective. We are waiting for a connector unit to get it working and will be testing its performance shortly afterwards.
A new 20x Plan Achromat VC objective (air) has arrived and is installed on the NIKON A1R/STORM machine. The multi immersion objective is removed from the turret but will remain available for users (ask CCI staff if you prefer to use it). The new objective is more widely applicable, has a working distance of 1 mm, a NA of 0.75 and should work well with the perfect focus system. We will test it in the coming period.
A BCN3D Sigma printer is now stationed at the CCI. It is a dual color (IDEX) 3D printer meant to manufacture (prototype) parts for the microscopes. Printing strategies and materials will be tested in the coming period.
After a long wait over the holiday period, the repair of the EL6000 fluorescence unit from the SPE2 LEICA confocal has been completed. It can be used again for finding fluorescence using the eyepieces before imaging on the CLSM.