We are grateful to the Veterinary Faculty Utrecht to support an upgrade for the NIKON A1R system. Many users will benefit from this upgrade since it will vastly improve confocal imaging speeds. The upgrade will be performed around the last week of May 2019.
Soledad Ordoñez-Alvarez has been appointed for 2 days a week as a new staff member of the CCI. She specializes in instructing new users and adds expertise in image analyses to the CCI.
Unfortunately, Rob Bleumink had to retire. Since we miss his expertise in cryosectioning (as for countless other issues), we moved the cryostate to the Nieuw Gildestein building in room O.53 under the expert guidance of Judith Hendriks (department Dier in Wetenschap en Maatschappij email@example.com). She will take care of instructions and access to the cryostate. Please contact her to arrange this.
The OMX has been serviced , the OTFs have been updated and saved as default for SIM reconstructions. A 10x objective is available now to perform tile scans for sample overviews. The 4th camera is non functional due to a power failure, repair parts are on order. So for now, the far red detection is not possible.
A newly developed joystick is installed on the OMX-V4, it allows navigating through a sample, mark and start acquisition/mosaic. It is a bit fast but with practice will help you find the best cells more efficiently.
Few new features are implemented during the last service visit (for example: laser power tuning), ask CCI staff to inform you before working on the machine. Again, be sure to keep the OTFs of April 2018 for the reconstructions.
The TOKAI HIT heater for lenses on the NIKON system is repaired. Please be careful wrapping the foil around the lenses, the fit should be tight but do not pull the wiring or deform the aluminium foil while wrapping.
The OMX system has been serviced and new OTFs have been generated. The current OTF set that is selected in SoftWoRx should be used for reconstructions, do not change it!
Unfortunately, the TOKAI hit lens heater is broken again. We are in contact with the Japanese supplier but it may take some time for it to be repaired (it needs to be sent to Japan). We have imaged live cells without the heater overnight without any problems, yet the temperature at the lens spot will be several degrees below the set temperature. Contact CCI staff if you intent to install the hairdryer for additional heating.
The TOKAI hit objective heater is complete again. Be careful when changing objectives when the velcro is around it. The connecting wires can be dislodged from the connectors by these fast movements. Also, the aluminium inner surface is fragile be carefull when you wrap te velcro around an objective.
The objective heater for live cell imaging on the NIKON A1R-STORM system is broken. The thermocouple is being replaced. For short term time lapse (and vibration insensitive imaging) you can use the hair dryer to warm the lower part of the microscope.