We are grateful to the Veterinary Faculty Utrecht to support an upgrade for the NIKON A1R system. Many users will benefit from this upgrade since it will vastly improve confocal imaging speeds. The upgrade will be performed around the last week of May 2019.
Soledad Ordoñez-Alvarez has been appointed for 2 days a week as a new staff member of the CCI. She specializes in instructing new users and adds expertise in image analyses to the CCI.
Unfortunately, Rob Bleumink had to retire. Since we miss his expertise in cryosectioning (as for countless other issues), we moved the cryostate to the Nieuw Gildestein building in room O.53 under the expert guidance of Judith Hendriks (department Dier in Wetenschap en Maatschappij firstname.lastname@example.org). She will take care of instructions and access to the cryostate. Please contact her to arrange this.
BioImaging Utrecht will have a winter meeting on Wednesday 21st of February from 10:00 to 11:00. It will be followed by an IMARIS workshop at the Stratenum (MCR). Georgia Golfis will outline the program at 11:00 -12:30 (Rm STR3.108) and she will train groups to work with own datasets in the afternoon. The next day, Thursday the 22nd a workshop to all Imaris users will be given (RM STR 2.106). Registration for the workshop is required by mail to Livio (L.Kleij@umcutrecht.n). Highly recommended for Imaris users!! (Announcement PDF).
At the CCI office, a slide and photo scanner (link) was installed to scan negatives, overviews of histology slides and exposed ECL films. Please contact Richard if you want to use it.
The BioImaging Utrecht community (link) has a number of events planned that are highly recommended.
Hardware Microscopy demonstrations.
Demonstration of the re-scan confocal (link) at the UMCU was only last week (contact Corlinda if you want to know how good it was). Additional product demonstrations are planned:
29th of September:
CeniBra / Yokogawa representatives will give talks on a spinning disk desktop machine, the CQ1 (link) that should be well suited to image and analyse organoid containing volumes.
4th of October
Leica will host lectures (link) on their high end imaging systems on the 4th of October (especially their new DMi8S TIRF live imaging setup) – Lukas Kapitein and Philip Tinnefeld will lecture on TIRF use in their labs, no doubt spectacular work will be shown. Hands-on workshops will continue the next day.
The Utrecht Image Contest (link)
This year Utrecht microscopists are again invited to send in their most beautiful and/or artistic image in jpg format to compete for the prestigious title of the best Utrecht Microscopy Image in the Electron Microscopy or Fluorescence microscopy categories. In addition, a printout by pArt of Science (link) and a prize of 200 Euro (provided by Carl Zeiss) can be won. Please supply image with microscope information and a brief scientific description to : email@example.com
ImageJ/FIJI workshop (BioImaging Utrecht_ImageJ beginners 2017)
At this workshop, image analyses experts in the Utrecht imaging community will train you to learn using ImageJ/FIJI and give you an idea how to start analyzing (microscopy) images in general. Highly recommended for microscopists. Good to realize that image analyses will usually make your image acquisition better and will help you focus on the actual measurement of imaging process. Registration deadline October 1st (ImageJ_beginners_registration form 2017).
The theme of the upcoming spring meeting of the BioImaging Utrecht society is: time resolved 3D imaging of large samples. Please find the announcement poster (here) to print and display. Presentations on using light sheet microscopy in brain development and introducing Anne Rios’ work on imaging the 3D architecture of developing breast cancers.
Place: David de Wied building Rm M2.01
Date and time: Tuesday 16th of May at 15:00.
Finally, we have curtains around the SPEII to seal the microscope space from the rest of the lab. This way we now can work normally in the wetlab again with the lights on.
Roos Masereeuw (Dept Farmacy) and Bart Gadella (Fac Vet Med) took the initiative to arrange a kick-off meeting for a consortium within the UU community to combine expertise on microfluidic device fabrication and developments in (combined) organ(s) on a chip. The FVM dean, Wouter Dhert (who represents the joint life sciences faculties) gave a highly motivated talk to get this initiative started to: 1) get better disease models to test treatments, 2) reduce animal use for drug testing, 3) better model the complexity of (human) disease. In different animal models, Bart Gadella explained that correct in vivo fertilization in a fabricated mold that was coated with differentiated oviduct epithelia resemble replanted fertilized embryos efficiencies better (i.e. less polyspermia and better early embryonal development) than standard IVF. Roos Masereeuw explained her work on renal proximal tubuli endothelia in different biofabricated support systems to each measure aspects of the renal transport processes. She stressed that existing animal model systems have relatively poor predictable value for renal toxicity as is clear from experimental drugs that are rejected in the later phases of clinical trials. OOC systems have a great promise to outperform animal testing models. Pedro Costa explained the capabilities of the biofabrication facility at the RMCU to design (in a user friendly interface) and manufacture biological culture models in complex structural design and in different biological sample mixtures.
In the discussion, Roos noted the Brain-on-a-Chip meeting that is planned in next week (link). A regular meetup of the UU initiative will be planned and a questionnaire to monitor interests in the UU community is going to be circulated. Collaboration with a national intiative (hdmt) will be investigated .
The BioImaging community in Utrecht has its quarterly meeting on Tuesday November the 29th. Gerhard Blab will talk about the possibilities and mistakes in image analysis anddifferent microscope manufacturers will talk about and demo small microscopes in the Stratenum Building. At the end, the Imaging contest winners will be announced. <flyer>
Date: November 29th 2016
Location: UMCU, Stratenum Building. Vondelzaal (Rm. 3.108)
Time: 15:00-17:00 (+drinks)