On Tuesday 28th of February to Wednesday the 1st of March the OMX will be unavailable. A sCMOS PCO camera will be replaced and the system will be fully re-aligned.
The OMX has been serviced and new OTFs have been made. We recommend using these. A strange bug in the acquisition software causes the 405 nm laser line (1st channel) to present wrong values, i.e. intensity % of the laser in the drop-down list do not represent the actual values. The desktop background picture of the PC instructs you restore the right interface (in 3 clicks).
Our new 63x lens (NA 1.2) is working from within ZEN black. A slider becomes available when you choose the objective that allows adjusting the depth correction of the lens. It is very nice to get a better psf at the depth of your most interesting feature. Good when you plan to deconvolve images post acquisition.
Finally, we have curtains around the SPEII to seal the microscope space from the rest of the lab. This way we now can work normally in the wetlab again with the lights on.
We are very grateful to the faculty to expand the Zeiss LSM880 NLO functionality with a hi NA (1.2, 63x) water immersion objective with an automated correction collar. This will allow high resolution imaging on the machine that is currently equipped with a 20x (NA 0.8) and a 32x (NA 0.85) objective. We are waiting for a connector unit to get it working and will be testing its performance shortly afterwards.
A new 20x Plan Achromat VC objective (air) has arrived and is installed on the NIKON A1R/STORM machine. The multi immersion objective is removed from the turret but will remain available for users (ask CCI staff if you prefer to use it). The new objective is more widely applicable, has a working distance of 1 mm, a NA of 0.75 and should work well with the perfect focus system. We will test it in the coming period.
Happy 2017. May this year bring you happiness, health, joy, strength and success.
Roos Masereeuw (Dept Farmacy) and Bart Gadella (Fac Vet Med) took the initiative to arrange a kick-off meeting for a consortium within the UU community to combine expertise on microfluidic device fabrication and developments in (combined) organ(s) on a chip. The FVM dean, Wouter Dhert (who represents the joint life sciences faculties) gave a highly motivated talk to get this initiative started to: 1) get better disease models to test treatments, 2) reduce animal use for drug testing, 3) better model the complexity of (human) disease. In different animal models, Bart Gadella explained that correct in vivo fertilization in a fabricated mold that was coated with differentiated oviduct epithelia resemble replanted fertilized embryos efficiencies better (i.e. less polyspermia and better early embryonal development) than standard IVF. Roos Masereeuw explained her work on renal proximal tubuli endothelia in different biofabricated support systems to each measure aspects of the renal transport processes. She stressed that existing animal model systems have relatively poor predictable value for renal toxicity as is clear from experimental drugs that are rejected in the later phases of clinical trials. OOC systems have a great promise to outperform animal testing models. Pedro Costa explained the capabilities of the biofabrication facility at the RMCU to design (in a user friendly interface) and manufacture biological culture models in complex structural design and in different biological sample mixtures.
In the discussion, Roos noted the Brain-on-a-Chip meeting that is planned in next week (link). A regular meetup of the UU initiative will be planned and a questionnaire to monitor interests in the UU community is going to be circulated. Collaboration with a national intiative (hdmt) will be investigated .
The BioImaging community in Utrecht has its quarterly meeting on Tuesday November the 29th. Gerhard Blab will talk about the possibilities and mistakes in image analysis anddifferent microscope manufacturers will talk about and demo small microscopes in the Stratenum Building. At the end, the Imaging contest winners will be announced. <flyer>
Date: November 29th 2016
Location: UMCU, Stratenum Building. Vondelzaal (Rm. 3.108)
Time: 15:00-17:00 (+drinks)
Jingchao Wu published the first paper with image data using the OMX SIM setup. Congratulations to him and the co-authors. (Pubmed)