Handouts:
Quick reference (PDF) / NIS Elements 4.5  /  TIRF  / A1Rsi  (pdf’s available on  workstation and acquisition PC)  / Koehler alignment (PDF) / STORM procedures (PDF) / JOBS reference card (PDF)

Image Recording Units: 

• A1R confocal scanning unit with spectral 32 PMT detector array (left port)
• Andor iXon Ultra897 EM-CCD  camera for wide field/TIRF image recording (right port)

Special Add-ons:
– Diode lasers:

• 405 nm (100 mW) Coherent Viotet Cube
• 488 nm (200 mW) Saphire Blue Coherent
• 561 nm (100 mW) Sapphire Yellow Coherent
• 645 nm (300 mW) MPBC

– Lumencor Spectra X LED illumination for wide field imaging (fast wavelength changing in triggered mode via NIDAQ controller), experienced user manual is here.

• 395/25 nm
• 440/20 nm
• 470/24 nm
• 510/15 nm
• 550/15 nm*
• 575/25 nm*
• 640/30 nm

(*) one mounted at a time, exchange for optimal excitation

-Tokai Hit table top imaging chamber
– Accurate Focus control (Mad City Labs piezo stage and internal Nikon stage control via PFS)
– A1R resonance scanner to perform CLSM imaging (512×512) up to 30 fps
– GaAs-PMT(2x) for CLSM imaging
– spectral detector unit for CLSM imaging

Software to open RAW (.ND2) files

– FUJI/IJ with the BIOLOCI Importer (see link)
– NIS elements viewer (link)

Recording forms:
– Different webforms provide possibilities to log settings and supply notes to a mail. Checklists are also supplied on this site.

Speciality:
-Live cell (TIRF / Wide field and CLSM)
-N/d/c-STORM superresolution

Available lenses:  

*available but not standardly mounted (contact staff)

Videolinks: 
Excellent instructions to use NIS elements by Dr. Sylvie Le Guyader and Dr. Gabriela Imreh of the Karolinska Institutet, Sweden (link, 18:32 mins) and to learn the basics of image analysis in NIS elements (link, 27:19 mins).

Videos for Ti setup (mic stand), Kohler aligning and DIC alignment from NTC at the Carnegie Mellon University of Pittsburg.