Category Archives: NIKON A1R/TIRF/STORM

April 4: ImageJ Advanced workshop

The Bio-Imaging Utrecht community organizes an ImageJ/FIJI workshop for experienced users on Tuesday the 4th of April. Previous sessions were very well appreciated and they allow you to interact with experts in IJ coding. You can learn image analyses automation and the experts can help you find, tweak and apply the proper plugin(s) for you specific question. It is highly advised for microscopists who use FIJI but have problems overseeing the large analyses possibilities offered by the platform. Register using the form attached here (deadline 17th March) 

New NIKON 20x Plan Achromat objective

A new 20x Plan Achromat VC objective (air) has arrived and is installed on the NIKON A1R/STORM machine. The multi immersion objective is removed from the turret but will remain available for users (ask CCI staff if you prefer to use it). The new objective is more widely applicable, has a working distance of 1 mm, a NA of 0.75 and should work well with the perfect focus system. We will test it in the coming period.

Update NIS elements and STORM module

Today, the NIS elements software on the NIKON A1R/STORM system has been updated to the newest version for the NIS suite (4.5) and the STORM module (4.1). In STORM,  Z stacking features, fiducial marker integration and sequential recordings on identical positions can be performed, potentially on regions in  live cells. In addtition, the 405 laser was re-aligned and set at a higher level in the startup script to allow better imaging in the UV region. Users should take care that this laser setting is different from their previous sessions.

NIKON A1R STORM TIRF/NIDAQ triggering

The TIRF module experienced a problem yesterday and was repaired this morning. All is functional again.

The NIDAQ triggering of the EM-CCD camera no longer needs manual switching of BNC connectors, NIKON made a connector box that allows automated switching from within NIS elements. So triggering via the lasers (LU4) or wide field illumination (SpectraX) can be programmed within a ND acquisition procedure, useful for example in wide field imaging while performing FRAP or photoactivation.

DATA storage testing

A StorSimple backup system has been  implemented by ITS.  We have tested it for a couple of months and it seems to perform well. The extra network drive is available on all imaging machines (except for the Zeiss NLO system, that will be connected to the net in the coming weeks) and can be found at O:\Research\CCI\#equipment\##user##.  Writing on the drive  can only be done on our machines, users will be able to read only from these drives so data will be available but secured. We are implementing a secure logon system for users to read data from this drive.

 

New screws for the TOKAI HIT chamber holder

The screws of the Tokai hit chamber holder did not tighten well anymore. Focus shifts occurred when accurate imaging was required on live cells. It was already tedious to unscrew and rescrew the adapters for the different chambers and this has only become more cumbersome, unfortunately. We need to fasten the new screws and tighten them with the nuts below the stage while manoeuvring between the objectives, better done by slim finger persons. It works for now but we will improve this. If you ideas to make it easier, please inform the staff.